AM Normal Trisomy Cell Free gDNA

 

(Trisomy Cell Free gDNA)

AM Normal cfgDNA-1
Description: DNA is extracted and fragmented from a popular normal male cell line

Dissolved in TE: Part Number: 60133001
10ug per vial
Spiked in Plasma: Part Number: 60133501
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDNA-2
Description: DNA is extracted and fragmented from normal female cell line, PAM3005
Dissolved in TE: Part Number: 60133002
10ug per vial
Spiked in Plasma: Part Number: 60133502
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDNA-3
Description: DNA is extracted and fragmented from pooled normal peripheral blood mononuclear cells (PBMC) from fresh blood
Dissolved in TE: Part Number: 60133003
10ug per vial

(Trisomy Cell Free gDNA)
Spiked in Plasma: Part Number: 60133503
Pre-spiked with 250ng gDNA per 5ml

(Trisomy Cell Free gDNA)

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

AM Variant Trisomy Cell Free gDNA

 

(Trisomy Cell Free gDNA)

AM Multiplexed ctDNA
Description: DNA contains multiplexed 20+ cell-derived variants each at 5% Allele Frequency. DNA is extracted and fragmented from mutated PAM3005 cells

     Dissolved in TE: Part Number: 60135001
     10ug per vial
     Spiked in Plasma: Part Number: 60135501
     Pre-spiked with 250ng gDNA per 5ml

Variants included

APC Q1429*	KIT D816V
APC R1450*	KRAS G12C
BRAF V600E	KRAS G12D
CTNNB1 T41A	KRAS G12V
EGFR C797S	NRAS Q61R
EGFR E746-A750del	PIK3CA E545K
EGFR L858R	PIK3CA H1047R
EGFR D770_N771insG	PTEN R130G
EGFR T790M	TP53 R175H
FGFR3 S249C	TP53 R248Q
IDH1 R132H

AM Trisomy cfgDNA
Description: DNA is extracted and fragmented from cells harboring trisomy 13, 18 or 21. It is used for PCR- or counting-based NIPT assay (Trisomy Cell Free gDNA)
      Dissolved in TE: Part Numbers: 60134001 (Trisomy13), 60134002 (Trisomy18), 60134003 (Trisomy21)
     10ug per vial
     Spiked in Plasma: Part Numbers: 60134501 (Trisomy13), 60134502 (Trisomy18), 60134503 (Trisomy21)
     Pre-spiked with 250ng gDNA per 5ml

(Trisomy Cell Free gDNA)

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

ctDNA Controls & Reference Standards (Trisomy Cell Free gDNA)

 

(Trisomy Cell Free gDNA)

(Trisomy Cell Free gDNA)

Buffer-based or plasma-based controls are produced by mixing single or multiplexed mutant gene fragments with a native, whole-genome cfDNA background (Figure B).

Anchor Molecular manufactures two types of products: Mutation Quantification Control and Mutation Reference Standard. The user can quantify either the absolute copy numbers or the relative number of the mutant gene fragments.

AM Multiplexed Mutation Quantification Control

• Function as both positive and negative controls
• For use in daily assays
• AF% determined based on average wild-type copy numbers
• Customized controls available upon request
• Price upon request
• (Trisomy Cell Free gDNA)

This product is used as Positive and Negative Controls in daily assays. The AF% is determined based on average wildtype copy numbers. It includes all mutant genes listed in the table below. Custom products with different parameters are available upon request.

Cat. No.	Base Matrix (vol)	Allele Frequency (%)	Wildtype gDNA (ng)
60100101	Buffer (25 ul)	0 or 2	250
60100102	EDTA Plasma (5 ml)	0 or 2	50
60100103	EDTA Plasma (5 ml)	0 or 2	25

AM Multiplexed Mutation Reference Standard

• Highest quality reference standards
• For use in research and assay development
• Mutant genes at fixed allele frequency (AF%) determined by ddPCR
• Customized reference standards available upon request
• Price upon request

Mutant genes are made at fixed allele frequency (AF%) determined by ddPCR. The product includes all mutant 37 mutant genes listed in red in the table below. Custom products with different parameters are available upon request.

Cat. No.	Base Matrix (vol)	Allele Frequency (%)	Wildtype gDNA (ng)
60100201	Buffer (25 ul)	0, 0.1, 0.3, 1, or 2	250
60100202	EDTA Plasma (5 ml)	0, 0.1, 0.3, 1, or 2	125

(Trisomy Cell Free gDNA)

Mutation List  

EGFR (T790M)	KRAS (G12S)	TP53 (R175H)	EGFR (D770-N771insG)
EGFR (C797S)	KRAS (G12A)	APC (R1450X)	EGFR (delE746-A750)
EGFR (T790M & C797S cis)	KRAS  (G12R)	Beta2-M	EGFR (delL747-T751insS)
EGFR (G719S)	KRAS (G13D)	DDR2	EGFR (delL747-P753insS)
EGFR (G719C)	BRAF (V600E)	LKB1
EGFR (G719A)	BRCA1 (P871L)	MDM2
EGFR (S720F)	BRCA2 (N372H)	MDM4	EML4-ALK v1
EGFR (L858R)	ERBB2 (S310F)	POLE	EML4-ALK v2
EGFR (L861Q)	JAK2 (V617F)	PTEN	EML4-ALK v3
EGFR (L861R)	MAP2K1 (P124S)	SKT11	CCDC6-RET
KRAS (G12D)	MET (Y1253D)		NCOA4-RET
KRAS (G12V)	PIK3CA (H1047R)	EGFR (D770-N771insNPG)	CD74-ROS1
KRAS (G12C)	TERT (A305A)	EGFR (D770-N771insSVQ)	SLC34A2-ROS1